Complete sequencing of an IncH plasmid carrying the blaNDM-1, blaCTX-M-15 and qnrB1 genes.
Identifieur interne : 000154 ( Main/Exploration ); précédent : 000153; suivant : 000155Complete sequencing of an IncH plasmid carrying the blaNDM-1, blaCTX-M-15 and qnrB1 genes.
Auteurs : Laura Villa [Italie] ; Laurent Poirel ; Patrice Nordmann ; Claudio Carta ; Alessandra CarattoliSource :
- The Journal of antimicrobial chemotherapy [ 1460-2091 ] ; 2012.
Descripteurs français
- KwdFr :
- ADN bactérien (composition chimique), ADN bactérien (génétique), Analyse de séquence d'ADN (MeSH), Conjugaison génétique (MeSH), Données de séquences moléculaires (MeSH), Escherichia coli (génétique), Humains (MeSH), Infections à Klebsiella (microbiologie), Klebsiella pneumoniae (génétique), Maroc (MeSH), Plasmides (MeSH), Protéines bactériennes (génétique), Variation génétique (MeSH), bêta-Lactamases (génétique).
- MESH :
- composition chimique : ADN bactérien.
- génétique : ADN bactérien, Escherichia coli, Klebsiella pneumoniae, Protéines bactériennes, bêta-Lactamases.
- microbiologie : Infections à Klebsiella.
- Analyse de séquence d'ADN, Conjugaison génétique, Données de séquences moléculaires, Humains, Maroc, Plasmides, Variation génétique.
- Wicri :
- geographic : Maroc.
English descriptors
- KwdEn :
- Bacterial Proteins (genetics), Conjugation, Genetic (MeSH), DNA, Bacterial (chemistry), DNA, Bacterial (genetics), Escherichia coli (genetics), Genetic Variation (MeSH), Humans (MeSH), Klebsiella Infections (microbiology), Klebsiella pneumoniae (genetics), Molecular Sequence Data (MeSH), Morocco (MeSH), Plasmids (MeSH), Sequence Analysis, DNA (MeSH), beta-Lactamases (genetics).
- MESH :
- chemical , chemistry : DNA, Bacterial.
- chemical , genetics : Bacterial Proteins, DNA, Bacterial, beta-Lactamases.
- geographic : Morocco.
- genetics : Escherichia coli, Klebsiella pneumoniae.
- microbiology : Klebsiella Infections.
- Conjugation, Genetic, Genetic Variation, Humans, Molecular Sequence Data, Plasmids, Sequence Analysis, DNA.
Abstract
OBJECTIVES
To characterize plasmid pNDM-MAR recovered from a Klebsiella pneumoniae isolate of sequence type (ST) 15 from Morocco, carrying the genes encoding the metallo-β-lactamase NDM-1, the extended-spectrum β-lactamase (ESBL) CTX-M-15 and the qnrB1 quinolone resistance determinant.
METHODS
The plasmid DNA sequence was obtained by using the 454-Genome Sequencer FLX procedure on a library constructed from total plasmid DNA obtained from an Escherichia coli J53 transconjugant. Contig assembly and predicted gaps were confirmed and filled by PCR-based gap closure.
RESULTS
Plasmid pNDM-MAR was 267 242 bp long and encoded 177 predicted proteins. It harboured novel replicons and transfer loci, defining a novel plasmid type within the IncH plasmid family. The bla(NDM-1) gene was flanked by genetic elements that are distinct from those observed in other bla(NDM-1)-positive plasmids, including the groES and groEL chaperonin genes. This plasmid harboured the ESBL gene bla(CTX-M-15) together with the quinolone resistance gene qnrB1. In addition, it harboured genes encoding resistance to tellurite, mercury, chloramphenicol and aminoglycosides. Interestingly, pNDM-MAR did not carry any 16S rRNA methylase gene, in contrast to other bla(NDM-1)-positive plasmids.
CONCLUSIONS
This study underlines the diversity of genetic vehicles involved in the spread of the bla(NDM-1) gene. Plasmid pNDM-MAR differed significantly from all known bla(NDM-1)-bearing plasmids. Comparative analysis of the pNDM-MAR sequence identified a novel type of IncH plasmid.
DOI: 10.1093/jac/dks114
PubMed: 22511638
Affiliations:
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Le document en format XML
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last="Nordmann">Patrice Nordmann</name></author><author><name sortKey="Carta, Claudio" sort="Carta, Claudio" uniqKey="Carta C" first="Claudio" last="Carta">Claudio Carta</name></author><author><name sortKey="Carattoli, Alessandra" sort="Carattoli, Alessandra" uniqKey="Carattoli A" first="Alessandra" last="Carattoli">Alessandra Carattoli</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22511638</idno><idno type="pmid">22511638</idno><idno type="doi">10.1093/jac/dks114</idno><idno type="wicri:Area/PubMed/Corpus">000668</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000668</idno><idno type="wicri:Area/PubMed/Curation">000664</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000664</idno><idno type="wicri:Area/PubMed/Checkpoint">000670</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" 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Laurent" uniqKey="Poirel L" first="Laurent" last="Poirel">Laurent Poirel</name></author><author><name sortKey="Nordmann, Patrice" sort="Nordmann, Patrice" uniqKey="Nordmann P" first="Patrice" last="Nordmann">Patrice Nordmann</name></author><author><name sortKey="Carta, Claudio" sort="Carta, Claudio" uniqKey="Carta C" first="Claudio" last="Carta">Claudio Carta</name></author><author><name sortKey="Carattoli, Alessandra" sort="Carattoli, Alessandra" uniqKey="Carattoli A" first="Alessandra" last="Carattoli">Alessandra Carattoli</name></author></analytic><series><title level="j">The Journal of antimicrobial chemotherapy</title><idno type="eISSN">1460-2091</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial Proteins (genetics)</term><term>Conjugation, Genetic (MeSH)</term><term>DNA, Bacterial (chemistry)</term><term>DNA, Bacterial (genetics)</term><term>Escherichia coli (genetics)</term><term>Genetic Variation (MeSH)</term><term>Humans (MeSH)</term><term>Klebsiella Infections (microbiology)</term><term>Klebsiella pneumoniae (genetics)</term><term>Molecular Sequence Data (MeSH)</term><term>Morocco (MeSH)</term><term>Plasmids (MeSH)</term><term>Sequence Analysis, DNA (MeSH)</term><term>beta-Lactamases (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (composition chimique)</term><term>ADN bactérien (génétique)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Conjugaison génétique (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Escherichia coli (génétique)</term><term>Humains (MeSH)</term><term>Infections à Klebsiella (microbiologie)</term><term>Klebsiella pneumoniae (génétique)</term><term>Maroc (MeSH)</term><term>Plasmides (MeSH)</term><term>Protéines bactériennes (génétique)</term><term>Variation génétique (MeSH)</term><term>bêta-Lactamases (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>DNA, Bacterial</term><term>beta-Lactamases</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Morocco</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>ADN bactérien</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term><term>Klebsiella pneumoniae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN bactérien</term><term>Escherichia coli</term><term>Klebsiella pneumoniae</term><term>Protéines bactériennes</term><term>bêta-Lactamases</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Infections à Klebsiella</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Klebsiella Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Conjugation, Genetic</term><term>Genetic Variation</term><term>Humans</term><term>Molecular Sequence Data</term><term>Plasmids</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Conjugaison génétique</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Maroc</term><term>Plasmides</term><term>Variation génétique</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Maroc</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>OBJECTIVES</b></p><p>To characterize plasmid pNDM-MAR recovered from a Klebsiella pneumoniae isolate of sequence type (ST) 15 from Morocco, carrying the genes encoding the metallo-β-lactamase NDM-1, the extended-spectrum β-lactamase (ESBL) CTX-M-15 and the qnrB1 quinolone resistance determinant.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>The plasmid DNA sequence was obtained by using the 454-Genome Sequencer FLX procedure on a library constructed from total plasmid DNA obtained from an Escherichia coli J53 transconjugant. Contig assembly and predicted gaps were confirmed and filled by PCR-based gap closure.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Plasmid pNDM-MAR was 267 242 bp long and encoded 177 predicted proteins. It harboured novel replicons and transfer loci, defining a novel plasmid type within the IncH plasmid family. The bla(NDM-1) gene was flanked by genetic elements that are distinct from those observed in other bla(NDM-1)-positive plasmids, including the groES and groEL chaperonin genes. This plasmid harboured the ESBL gene bla(CTX-M-15) together with the quinolone resistance gene qnrB1. In addition, it harboured genes encoding resistance to tellurite, mercury, chloramphenicol and aminoglycosides. Interestingly, pNDM-MAR did not carry any 16S rRNA methylase gene, in contrast to other bla(NDM-1)-positive plasmids.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>This study underlines the diversity of genetic vehicles involved in the spread of the bla(NDM-1) gene. Plasmid pNDM-MAR differed significantly from all known bla(NDM-1)-bearing plasmids. Comparative analysis of the pNDM-MAR sequence identified a novel type of IncH plasmid.</p></div></front></TEI><affiliations><list><country><li>Italie</li></country><region><li>Latium</li></region><settlement><li>Rome</li></settlement></list><tree><noCountry><name sortKey="Carattoli, Alessandra" sort="Carattoli, Alessandra" uniqKey="Carattoli A" first="Alessandra" last="Carattoli">Alessandra Carattoli</name><name sortKey="Carta, Claudio" sort="Carta, Claudio" uniqKey="Carta C" first="Claudio" last="Carta">Claudio Carta</name><name sortKey="Nordmann, Patrice" sort="Nordmann, Patrice" uniqKey="Nordmann P" first="Patrice" last="Nordmann">Patrice Nordmann</name><name sortKey="Poirel, Laurent" sort="Poirel, Laurent" uniqKey="Poirel L" first="Laurent" last="Poirel">Laurent Poirel</name></noCountry><country name="Italie"><region name="Latium"><name sortKey="Villa, Laura" sort="Villa, Laura" uniqKey="Villa L" first="Laura" last="Villa">Laura Villa</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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